A new site-specific endonuclease from Streptomyces lavendulae (SlaI) |
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Authors: | Hideo Takahashi Mariko Shimizu Hiuga Saito Yonosuke Ikeda Hiroyuki Sugisaki |
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Institution: | 1. Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo Japan;2. Institute for Chemical Research, Kyoto University, Uji, Kyoto-fu Japan |
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Abstract: | A new restriction-like endonuclease, SlaI, was found and partially purified from Streptomyces lavendulae ATCC8664. This endonuclease cleaved bacteriophage lambda DNA at only one site, and cytosine-substituted bacteriophage T4 DNA at 16 sites. The recognition sequence was determined by using SlaI fragments of cytosine-substituted bacteriophage T4 DNA. The hexanucleotide recognized by SlaI endonuclease was with the sites of cleavage as indicated by the arrows. Therefore, SlaI endonuclease was an isochizomer of XhoI endonuclease. |
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Keywords: | Bacteriophage λ CTCGAG recombinant DNA cytosine-substituted T4 DNA PEI polyethyleneimine |
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