| c-myc表达对顺铂诱导人胶质瘤细胞系BT_(325)凋亡的影响 |
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| 引用本文： | 张浩,王成济,杨安钢,曹云新,李玉松.c-myc表达对顺铂诱导人胶质瘤细胞系BT_(325)凋亡的影响[J].中国组织化学与细胞化学杂志,2000(3). |
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| 作者姓名： | 张浩 王成济 杨安钢 曹云新 李玉松 |
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| 作者单位： | 北京军事医学科学院基因工程研究室!100021(张浩)，第四军医大学基础部生物化学与分子生物学教研室!西安710032(王成济，杨安钢)，第四军医大学中心实验室!西安710032(曹云新)，第四军医大学病理学教研室!西安710032(李玉松) |
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| 摘 要： | 研究 c- m yc在化疗药物顺铂诱导肿瘤细胞凋亡中的作用。在原有已构建好 c- myc反义表达载体 pc DNA3- MYC并成功转染 BT32 5 细胞的基础上 ,用顺铂诱导细胞凋亡。采用 TUNEL技术并从光镜、电镜、荧光显微镜及激光共聚焦显微镜水平观察 BT32 5 细胞凋亡的形态学特征 ;流式细胞仪检测实验组 (即 pc DNA3- MYC转染的细胞 )及对照组 (包括未转染或仅转染空载体 pc DNA 3的细胞 )凋亡率的异同。结果显示 :与转染空载体 pc DNA3及未转染组的细胞相比 ,在转染 pc DNA 3-MYC的 BT32 5 细胞中 ,顺铂诱导的细胞凋亡作用明显受阻 ,结果表明 :肿瘤细胞内异常表达的 c- myc在顺铂诱导肿瘤细胞凋亡的过程中有重要作用。
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| 关 键 词： | 顺铂 细胞凋亡 BT_(325)细胞 反义表达载体 c-myc 人 |
THE EFFECT OF C-MYC EXPRESSION ON CISPLATIN-INDUCED-APOPTOSIS OF HUMAN GLIOMA CELL LINE BT_(325) |
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| Zhang Hao,Wang Chengji,Yang Angang,Cao Yunxin,Li Yusong.THE EFFECT OF C-MYC EXPRESSION ON CISPLATIN-INDUCED-APOPTOSIS OF HUMAN GLIOMA CELL LINE BT_(325)[J].Chinese Journal of Histochemistry and Cytochemistry,2000(3). |
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| Authors: | Zhang Hao Wang Chengji Yang Angang Cao Yunxin Li Yusong |
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| Institution: | Zhang Hao,Wang Chengji,Yang Angang,Cao Yunxin1,Li Yusong2 |
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| Abstract: | The purpose of this study was to examine the effect of c- myc expression on cisplatin- induced- apoptosis.A recombinanteukaryotic expression vectorof c- myc antisense( pc DNA3- MYC) was previously constructed and used to transfect human glioma cell line BT3 2 5.Cell apoptosis was induced by cisplatin. The morphological changes of apoptotic BT3 2 5cells were examined with light microscope,electron microscope,fluorescence microscope and laser confocal scanning microscopy.Then we detected the difference ofapoptotic cell percentage among the experimental and control cell groups.Compared with that in the control cell groups,the cisplatin- induced- apoptosis was greatly blocked in the pc DNA3- MYC transfected cell group.The deregulated expression of c- myc in tumors cells may play an important role in the anti- cancer drug inducing apoptosis. |
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| Keywords: | Cisplatin Apoptosis BT3 2 5cell Antisense expressing vector c- myc Human |
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