| Chelex-100快速提取放线菌DNA作为PCR扩增模板 |
| |
| 引用本文： | 周双清,黄小龙,黄东益,胡新文,陈吉良.Chelex-100快速提取放线菌DNA作为PCR扩增模板[J].生物技术通报,2010(2). |
| |
| 作者姓名： | 周双清 黄小龙 黄东益 胡新文 陈吉良 |
| |
| 作者单位： | 海南大学农学院,儋州,571737 |
| |
| 基金项目： | 海南省高等学校博士生创新科研课题 |
| |
| 摘 要： | 旨在建立有效扩增16S rRNA基因序列的放线菌DNA快速提取的方法。采用Chelex-100法提取放线菌DNA,使用PCR扩增16S rRNA基因序列评价提取核酸的质量。结果显示,Chelex-100法能够在10 min之内从放线菌中快速提取DNA,所提取的DNA可以直接用于PCR扩增反应,PCR扩增产物电泳条带清晰,符合理论预期结果。因此,Chelex-100法提取放线菌DNA可以作为16S rRNA基因序列PCR扩增的模板,该方法具有经济、简便、快速的特点,适合于放线菌菌株大规模地筛选和分类鉴定。
|
| 关 键 词： | 放线菌 DNA提取 |
A Rapid Method for Extracting DNA from Actinomycetes by Chelex-100 |
| |
| Zhou Shuangqing,Huang Xiaolong,Huang Dongyi,Hu Xinwen,Chen Jiliang.A Rapid Method for Extracting DNA from Actinomycetes by Chelex-100[J].Biotechnology Bulletin,2010(2). |
| |
| Authors: | Zhou Shuangqing Huang Xiaolong Huang Dongyi Hu Xinwen Chen Jiliang |
| |
| Institution: | Zhou Shuangqing Huang Xiaolong Huang Dongyi Hu Xinwen Chen Jiliang(Agronomy College,Hainan University,Danzhou 571737) |
| |
| Abstract: | To establish a rapid method for extracting DNA from actinomycetes,DNA was extracted from actinomycetes by Chelex-100.The quality of the extraction by 16S rRNA gene was evaluated with PCR technique.The PCR product of 16S rRNA gene was used as a mark fragment.Results showed the Chlex-100 can quickly extract the DNA from actinomycetes about in 10 min.The DNA can be directly applied to the following step-PCR amplification as DNA template.Electrophoresis results of the PCR products was clear.Therefore,Chelex-100... |
| |
| Keywords: | Chelex-100 PCR 16S rRNA Chelex-100 Actinomycetes DNA extraction PCR 16S rRNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
