Ac transposition in transgenic tomato plants |
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| Authors: | John I Yoder Joe Palys Kevin Alpert and Michael Lassner |
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| Institution: | (1) Department of Vegetable Crops, University of California-Davis, 95616 Davis, CA, USA;(2) Campbell Institute of Research and Technology, Route 1, Box 1314, 95616 Davis, CA, USA |
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| Abstract: | Summary As an initial step towards developing a transposon mutagenesis system in tomato, the maize transposable element Ac was transformed into tomato plants via Agrobacterium tumefaciens. Southern analysis of leaf tissue indicated that in nine out of eleven transgenic plants, Ac excised from the T-DNA and reintegrated into new chromosomal locations. The comparison of Ac banding pattern in different leaves of the same primary transformant provided evidnece for transposition during later stages of transgenic plant development. There was no evidence of Ds mobilization in tomato transformants. |
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| Keywords: | Transposon-tagging Lycopersicon esculentum Ac element Gene transfer |
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