Measurement of ethanol concentration using solvent extraction and dichromate oxidation and its application to bioethanol production process |
| |
Authors: | Hyun-Beom Seo Hyun-Joo Kim Oh-Kyu Lee Ji-Hye Ha Hyeon-Yong Lee Kyung-Hwan Jung |
| |
Institution: | (1) Division of Food and Biotechnology, Chungju National University, Jeungpyung, Chungbuk, 368-701, Republic of Korea;(2) School of Liberal Arts, Chungju National University, Chungju, Chungbuk, 380-702, Republic of Korea;(3) Korea Forest Research Institute, Seoul, 130-712, Republic of Korea;(4) College of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Republic of Korea;(5) Research Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Republic of Korea; |
| |
Abstract: | A method for measuring the ethanol concentration in a yeast culture broth was developed using both microtubes and a 96-deepwell
microplate. The strategy involved first the solvent extraction of ethanol from the yeast culture broth and measurements of
the ethanol concentration using the dichromate oxidation method. Particular focus was made on selecting the extraction solvent
as well as determining the measurable range of ethanol concentrations using this solvent extraction-dichromate oxidation method.
This method was developed as an assay format in 2.0-ml microtubes and 1.2-ml 96-deepwell microplates, and the ethanol concentration
in the batch cultures and fed-batch fermentations was measured. Tri-n-butyl phosphate non-alcoholic solvent, density = 0.9727, solubility in water = 0.028% (w/v)] was used for solvent extraction
when measuring the ethanol concentration from the yeast culture broth. The maximum detectable ethanol concentration was 8%
(v/v) when 10 g potassium dichromate in 100 ml of 5 M sulfuric acid was used. The concentrations determined from the solvent
extraction-dichromate oxidation methods were remarkably similar to those of gas chromatography in which samples were prepared
from seven experiments, such as four batch cultures and three fed-batch fermentations. |
| |
Keywords: | Ethanol measurement Alcoholic fermentation Tri-n-butyl phosphate Dichromate Microtube 96-deepwell plate |
本文献已被 PubMed SpringerLink 等数据库收录! |
|