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| 球孢白僵菌Pr1蛋白酶基因cDNA克隆与序列分析 | |
| 引用本文： | 陈红梅,谢翎,吴甘霖,魏和平,黄勃.球孢白僵菌Pr1蛋白酶基因cDNA克隆与序列分析[J].生物学杂志,2010,27(6):61-64. |
| 作者姓名： | 陈红梅 谢翎 吴甘霖 魏和平 黄勃 |
| 作者单位： | 1. 安庆师范学院生命科学系,安庆,246011 2. 安庆师范学院生命科学系,安庆,246011;安徽农业大学微生物防治省重点实验室,合肥,230036 3. 安徽农业大学微生物防治省重点实验室,合肥,230036 |
| 基金项目： | 安庆师范学院科研启动基金 |
| 摘 要： | 从球孢白僵菌菌株Bb202中克隆了昆虫蛋白酶Pr1的基因。使用特异性引物,分别以基因组DNA和mRNA为模板进行PCR反应,得到了球孢白僵菌的Pr1蛋白酶基因cDNA和结构基因序列。测序结果表明球孢白僵菌Pr1蛋白酶结构基因全长大小为1606bp,含有3个内含子,分别为69、61和68bp。其ORF全长1140bp,预测编码379个氨基酸残基。成熟蛋白理论分子量为38.8kD,理论等电点为8.06。为进一步研究球孢白僵菌Pr1基因,并构建高毒力工程菌株提供了理论基础。 |
| 关 键 词： | 球孢白僵菌 Pr1基因 cDNA克隆 序列分析 |
Cloning and sequence analysis of Pr1 gene from Beauveria bassiana |
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| CHEN Hong-mei,XIE Ling,WU Gan-lin,WEI He-ping,HUANG Bo.Cloning and sequence analysis of Pr1 gene from Beauveria bassiana[J].Journal of Biology,2010,27(6):61-64. | |
| Authors: | CHEN Hong-mei XIE Ling WU Gan-lin WEI He-ping HUANG Bo |
| Institution: | 1.Department of Life Sciences,Anqing Teachers College,Anqing 246011;2.Anhui Provincial Key Laboratory of Microbial Pest Control,Anhui Agricultural University,Hefei 230036,China) |
| Abstract: | The Pr1 gene,a potential virulence factor with virtue of its activity against insect cuticles,was cloned from isolate Bb202 of Beuaveria bassiana.Result showed that the sequence of Pr1 gene was 1606 bp in full length.It included 3 introns which were 69,61 and 68 bp,respectively.The open reading frame（ORF） with 1140 bp encoded 379 amino acid.The mature protein had a molecular mass of 38.8 kD and pI of 8.06. |
| Keywords: | Pr1 |
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