灵芝多糖GLP 抑制小鼠骨肉瘤细胞S-180 在体内生长的作用机制

目的:探讨灵芝多糖成分(GLP)抑制肿瘤的作用机制。方法:在小鼠右腋皮下接种1×106TC-1细胞后7天后,用100mg/kg、200mg/kg和400mg/kg 3种剂量给小鼠口服灌胃给药20天,然后观察肿瘤的重量,并用ELISA检测小鼠血清中IL-2、IL-6和TNF-alpha,用流式细胞仪检测其外周血中CD4+和CD8+。结果:100mg/kg、200mg/kg和400mg/kg 3种剂量给小鼠口服灌胃给药20天,与对照组比较,抑瘤率分别可以达到53%、59%和58%,P<0.05;小鼠外周血血清中的IL-2从1.27ng/mL提高到了2.88ng/mL,P<0.05;TNF-α从1.05ng/mL提高到了1.82ng/mL,P<0.05;而IL-6则没有明显的变化。CD4+细胞水平升高(从54.80%提高到了58.27%),但差异无统计学意义(P>0.05);CD8+细胞明显增多(从24.15%提高到了45.36%),差异有统计学意义(P<0.05)。结论:GLP有明显抑瘤作用,但抑瘤作用与GLP剂量不存在依赖关系。GLP对肿瘤细胞生长的抑制是通过提高小鼠的细胞免疫能力来实现,而并非直接杀伤肿瘤细胞。

Inhibition of Ganoderma Lucidum Polysaccharides on Mouse Sarcoma S-180 Cell Proliferation in Vivo and its Mechanism

Objective: To investigate the effect of ganoderma lucidum polysaccharides(GLP) on mouse sarcoma S-180 cell prolif-eration in vivo and its mechanism.Methods: The mice were divided into 5 groups including saline control group,positive drug con-trolled group,treated groups(high,medium and low-dose group),the treated groups were treated with different dose of GLP(400,200 and 100 mg/kg,respectively).20 days later,the tumor weights were detected.The expression of CD4+ and CD8+ were assayed by flow cy-tometry.The concentrations of IL-2,IL-6 and TNF-alpha in serum were also assayed by ELISA.Results: Compared with that in the con-trol group,the inhibitory rate of high,medium and low-dose group was 58%,59% and 53%,respectively;The IL-2 were improved from 1.27 to 2.88ng/mL,TNF-α were improved from 1.05 to 1.82ng/mL,while there were no change in IL-6.CD8+ increased from 24.15% to 45.36 %(P<0.05),CD4+ increased significantly from 54.80% to58.27%(P>0.05).Conclusion: GLP can inhibit the growth of tumor,but the inhibiting effect has no relation with GLP dose.It was suggested that GLP inhibit the growth of tumor by improving cellular immunity instead of killing the tumor cells directly.