C-erBb-2、MMP-9 在结直肠癌中的表达及相关意义

目的:探讨C-erBb-2、基质金属蛋白酶-9(MMP-9)在结直肠癌中的表达与其浸润转移的关系,寻找浸润转移的客观指标及可能相关机制。方法:用免疫组化法测定80例结直肠癌组织中C-erBb-2和MMP-9的表达情况。结果:①C-erBb-2、MMP-9在结直肠癌组织中阳性表达率60.0%,(48/80)和56.2%(45/80)。②在有淋巴结转移的结直肠癌组织中C-erBb-2、MMP-9阳性率分别为75.0%(30/40)、72.5%(29/40),高于无淋巴结转移的结直肠癌组织中的C-erBb-2、MMP-9阳性率45%(18/30)、40.0%(16/40),(P<0.05);③二者共同阳性者淋巴结转移率为78.1%(25/32)高于二者均阴性者淋巴结转移率26.3%(5/19),(P<0.05),④C-erBb-2、MMP-9联合表达存在意义,且为正相关(r=0.257,P<0.05)。⑤C-erBb-2、MMP-9的表达与肿瘤的大小、性别、年龄、浸润层次等因素无关,但与肿瘤分期、淋巴结转移、分化有关。结论:C-erBb-2、MMP-9表达与淋巴结转移密切相关,二者在有淋巴结转移的结直肠癌组织中表达可能存在协同作用,可作为临床检测淋巴结转移的重要指标之一。

The Expression of C-erBb-2 and MMP-9 in Primary Colorectal Cancerand Clinical Significance

Objective: To investigate the expression of C-erBb-2 and MMP-9 in colorectal carcinoma and their relationship with lymph node transfer,and to investigate the transfer mechanism and the objective target.Methods: Immunohistochemical method was used to detect the expression of C-erBb-2 and MMP-9 in colorectal cancer tissues.Results:①The positive rates of C-erBb-2 and MMP-9 in tissues were 60.0 %(48/80) and 56.2 %(45/80).②The positive rates of C-erBb-2 and MMP-9 that with lymph node transfer were 75.0 %(30/40) and 72.5 %(29/40) which were higher than that with no lymph node transfer(the corresponding rates were 45 %(18/40) and 40.0 %(16/40))(P<0.05).③Two common positive rates with lymph node transfer were 78.1 %(25/32),which was higher than both nega-tive(26.3 %(5/19))(P<0.05).④The expression of C-erBb-2 and MMP-9 jointly existed significance,and there were positive correlations(r=0.257,P<0.05).⑤The expression of C-erBb-2 and MMP-9 had no relationship with the sizes of the tumor,gender and age,but had re-lationship with the lymph node transfer,differentiation and clinical stages.Conclusion: The expression of C-erBb-2 and MMP-9 had closely relationship with lymph node transfer in colorectal carcinoma,and the roles might synergetic,which could be used as one of the important clinical evidence that colorectal carcinoma with lymph node transfer.