Evidence for altered cell-cycle traverse of the non-modal cells of the heteroploid MCa-11 line |
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Authors: | W C Dooley D C Allison P Lin M Paul |
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Institution: | Department of Surgery, The Johns Hopkins Medical Institutions, Baltimore, Maryland;Department of Surgery, Medical College of Ohio, Toledo, Ohio, USA |
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Abstract: | Classic stem cell theory states that the growth of heteroploid cell populations is due to the proliferation of 'main stemline'cells with modal DNA content and chromosome number. Cells with non-modal DNA content and chromosome number are thought to be blocked and/or destroyed at mitosis. To test this, we studied two chromo-somally stable cell populations (mouse bone marrow and WCHE-5 cells) and one heteroploid, chromosomally diverse cell line (MCa-11). The heteroploid MCa-11 cells showed significant 3H]dT labelling for cells with DNA contents below the modal Go/G1 peak and above the modal G2 peaks ( P <0.001). This was consistent with the presence of cells with the non-modal DNA content that were engaged in replicative DNA synthesis. A percentage labelled mitosis analysis showed that MCa-11 cells with non-modal DNA content and chromosome number were able to complete mitosis, although with prolonged pre-karyokinetic time. These results suggest that many non-modal cells present in heteroploid cell populations are capable of continued proliferation. |
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