| 纤维素酶降解小麦秸秆最适条件的研究及其动力学分析 |
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| 引用本文： | 田萍,王浩菊,马齐,陈坤奇,周婷.纤维素酶降解小麦秸秆最适条件的研究及其动力学分析[J].氨基酸和生物资源,2012,34(2):13-15. |
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| 作者姓名： | 田萍 王浩菊 马齐 陈坤奇 周婷 |
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| 作者单位： | 西安理工大学应用化学系,陕西西安,710048;陕西省科学院酶工程研究所,陕西临潼,710600 |
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| 摘 要： | 以小麦秸秆为原料,通过正交实验对纤维素酶降解秸秆纤维的影响因素进行了研究。结果表明,影响小麦秸秆降解的因素依次为:酶量>酶解时间>料液比>反应温度,其最适条件是:加酶量为40u/g,酶解时间为10h,反应温度为40℃,料液比为1∶3,总糖含量达到43.24%。以米氏方程为基础,建立起最适酶解条件下总纤维素降解的动力学模型。
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| 关 键 词： | 小麦秸秆 纤维素酶 正交实验 动力学模型 |
Study on Optimal Conditions of Degradation of Wheat Straw by Cellulase and Analysis of Kinetics |
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| TIAN Ping,WANG Haoju,MA Qi,CHEN Kunqi,ZHOU Ting.Study on Optimal Conditions of Degradation of Wheat Straw by Cellulase and Analysis of Kinetics[J].Amino Acids & Biotic Resources,2012,34(2):13-15. |
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| Authors: | TIAN Ping WANG Haoju MA Qi CHEN Kunqi ZHOU Ting |
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| Institution: | 1. Department of Applied Chemistry, Xi＇an University of Technology, Xi＇an 710048, Shanxi, China 2. Enzyme Engineering Institute of Shaanxi Academy of Sciences, Lintong 710600, Shanxi, China) |
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| Abstract: | The influence factors of degradation of straw fiber by cellulase were studied using wheat straw as the raw material through the orthogonal experiments. The results showed that the factors of influence on enzymatic hydrolysis of wheat straw were successively： the amount of enzyme〉the time of hydrolysis〉the ratio of material and liquid〉reaction temperature. The optimal conditions of enzymatic hydrolysis were that the amount of cellulase was 40 u/g, the time of hydrolysis was 10 h, the reaction temperature was 40℃ and the ratio of material to liquid was 1：3. The total sugar contents could reach 43.24%. Under the optimal conditions, the kinetics model of degradation of the total cellulose was established based on Michaelis-Menten equation. |
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| Keywords: | wheat straw cellulase orthogonal experiment kinetics model |
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