抗菌肽人beta防御素3 对铜绿假单胞菌PAO-1 株的抑制作用

目的:分析抗菌肽人β防御素3(humanβdefensin 3,hBD3)对铜绿假单胞菌PAO-1株的抑制作用。方法:合成抗菌肽hBD3,分别通过最低抑菌浓度(minimal inhibitory concentration,MIC)检测、直接杀菌试验、重要功能基因检测分析其对PAO-1的直接抑制作用;并将其与阿奇霉素、四环素、利福平、氯霉素、链霉素、环丙沙星联合施用,观察对抗生素MIC的影响。结果:HBD3对PAO-1的MIC为32μg/mL;在浓度达到8μg/mL时即有明显杀菌作用。HBD3上调PAO-1株的ahpF基因表达,下调aprA和rhlR基因表达。在联用5μg/mL的hBD3后,四环素、利福平、氯霉素、链霉素、环丙沙星的MIC值均有降低。结论:抗菌肽hBD3对铜绿假单胞菌PAO-1株有显著的抑制作用。

The Inhibition on Pseudomonas Aeruginosa PAO-1 by Antimirobial Peptide Human beta Defensin 3

Objective:To analyze the inhibition effects of antimicrobial peptide human beta defensin 3 (hBD3) against Pseudomonas aeruginosa PAO-1.Methods:Antimicrobial peptide hBD3 was synthesized. The direct inhibition on PAO-1 was detected with the MIC (minimal inhibitory concentration) test, antibacterial activity test and the analysis on some important functional genes. The peptide was combined with azithromycin, tetracycline, rifampin, chloramphenicol, streptomycin, and ciprofloxacin for the effect on antibiotic MIC values.Results:The MIC of hBD3 against PAO-1 is 32 ug/mL, the bactericidal activity was observed while the hBD3 concentration as high as 8 ug/mL. The peptide hBD3 can improve the expression of ahpF and reduce the expression of aprA and rhlR. When 5 ug/mL peptide was added, the MIC values against PAO1 of tetracycline, rifampicin, chloramphenicol, streptomycin were dropped down.Conclusion:There is considerable bactericidal activity of antimicrobial peptide hBD3 against P. aeruginosa PAO1.