Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine. |
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| Authors: | G D Westrop K A Wareham D M Evans G Dunn P D Minor D I Magrath F Taffs S Marsden M A Skinner G C Schild et al. |
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| Affiliation: | Department of Microbiology, University of Reading, United Kingdom. |
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| Abstract: | The poliovirus type 3 Sabin oral poliovirus vaccine strain P3/Leon/12a1b differs in nucleotide sequence from its neurovirulent progenitor P3/Leon/37 by just 10 point mutations. The contribution of each mutation to the attenuation phenotype of the vaccine strain was determined by the construction of a series of recombinant viruses from infectious cDNA clones. The neurovirulence testing of recombinant viruses indicated that the attenuation phenotype is determined by just two point mutations: a C to U in the noncoding region at position 472 and a C to U at nucleotide 2034 which results in a serine-to-phenylalanine amino acid substitution in the structural protein VP3. |
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