A novel liver-directed gene delivery system using an autonomously replicating vector specifically expressed in AFP positive hepatoma cells |
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Authors: | YAN Ziying QIAO Jian GONG Huiyu HOU Yunde |
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Institution: | (1) State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, 100052 Beijing, China |
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Abstract: | A composite EBV-based expression vector, pEBAF, was constructed with the 5′-flanking sequence of human α-fetoprotein gene
as a promoter. Complexed to galactosylated histone, this vector with a foreign DNA insertion could be transferred into hepatic
ceils via the asialoglycoprotein receptor mediated endocytosis pathway. It was replicated as an episome, and its expression
was restricted to the AFP positive hepatoma cells. This new gene delivery method has the following advantages: (i) absence
of a potential toxicity is related to viral vectors; (ii) the targeting is specific to AFP positive hepatoma cells; (iii)
the introduction of the EBV replicon into this system results in the highlevel expression and long-term maintenance of the
transferred gene. This novel gene delivery system has potential applications in gene therapy for the treatment of hepatocellular
carcinoma.
Project supported by the National High Technology Development Program and partly by National Climbing Project. |
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Keywords: | EBV replicon vector AFP promoter receptor mediated gene transfer hepatoma cell |
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