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功率超声对地灵叶片愈伤组织诱导和芽生长的影响
引用本文:曾弦,肖娅萍,胡雅琴,张媛华.功率超声对地灵叶片愈伤组织诱导和芽生长的影响[J].广西植物,2004,24(2):130-133.
作者姓名:曾弦  肖娅萍  胡雅琴  张媛华
作者单位:陕西师范大学生命科学学院,陕西西安,710062
基金项目:教育部“利用生物技术保护与开发秦巴山区珍稀濒危药用植物资源研究”项目
摘    要:用频率分别为 2 7KHz和 40KHz的功率超声对地灵组培苗进行不同时间的处理 ,继代培养后进行观察。结果显示 :经超声处理的叶片形成愈伤组织时间比对照组提前 ,但在愈伤组织生长后期增殖与对照组没有差异 ;超声处理后的腋芽生长状况比对照组好 ,表现为腋芽高度增加 ,直径加粗 ,说明超声在一定程度上可以促进植物的生长。

关 键 词:功率超声  地灵  愈伤组织
文章编号:1000-3142(2004)02-0130-04
修稿时间:2003年7月10日

Effect of power ultrasound on induction of Stachys sieboldii leaf callus and growth of axillary bud
ZENG Xian,XIAO Ya-ping,HU Ya-qin,ZHANG Yuan-hua.Effect of power ultrasound on induction of Stachys sieboldii leaf callus and growth of axillary bud[J].Guihaia,2004,24(2):130-133.
Authors:ZENG Xian  XIAO Ya-ping  HU Ya-qin  ZHANG Yuan-hua
Institution:ZENG Xian,XIAO Ya-ping~*,HU Ya-qin,ZHANG Yuan-hua
Abstract:Using the power ultrasound 27 KHz and 40 KHz respectively to treat with Stachys sieboldii in different treatment time then the treated materials were sub cultured. We observed that the leaves callus formation time of ultrasound treatment groups was shortened comparing with the control ones. However, there was no apparent difference during the later growing period. The axillary buds of ultrasound treatment groups grow better than the control ones, such as increased height and thickened stem. It shows that power ultrasound, to some degree, could stimulate the growth of plant.
Keywords:power ultrasound  Stachys sieboldii  callus
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