PCR-based gene synthesis to produce recombinant proteins for crystallization |
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| Authors: | Damien Marsic Ronny C Hughes Miranda L Byrne-Steele Joseph D Ng |
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| Institution: | (1) ExtremoZyme Inc, HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA;(2) Laboratory for Structural Biology, Department of Biological Sciences, University of Alabama, Huntsville, AL 35899, USA |
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| Abstract: | Background Gene synthesis technologies are an important tool for structural biology projects, allowing increased protein expression through
codon optimization and facilitating sequence alterations. Existing methods, however, can be complex and not always reproducible,
prompting researchers to use commercial suppliers rather than synthesize genes themselves. |
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