Effects of NaCl and iso-osmotic PEG stress on growth,osmolytes accumulation and antioxidant defense in cultured sugarcane cells |
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Authors: | Vikas Yadav Patade Sujata Bhargava Penna Suprasanna |
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Institution: | (1) Functional Plant Biology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India;(2) Botany Department, University of Pune, Pune, 411007, India;(3) Present address: Molecular Biology and Genetic Engineering Division, Defence Institute of Bio-Energy Research, Goraparao, Haldwani, Nainital, 263139, India; |
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Abstract: | In order to discriminate between the ionic and osmotic components of salt stress, sugarcane (Saccharum officinarum L. cv. Co 86032) calli were cultured on media containing NaCl or polyethylene glycol (PEG) 8000 that exerted the same osmotic
pressure (−0.7 MPa). PEG stress exposure for 15 days led to significant growth reduction and loss in water content than salt
stressed and control tissues. Osmotic adjustment (OA) was observed in callus tissues grown on salt, but was not evident in
callus grown on PEG. Oxidative damage to membranes, estimated in terms of accumulation of thiobarbituric acid reactive substances-TBARS
and electrolytic leakage was significantly higher in both the stressed calli than the control however, the extent of damage
was more in the PEG stressed calli. The stressed callus tissues showed inhibition of ascorbate peroxidase activity, while
catalase activity was increased. These results indicate sensitivity of cells to PEG-mediated stress than salt stress and differences
in their OA to these two stress conditions. The sensitivity to the osmotic stress indicate that expression of the stress tolerance
response requires the coordinated action of different tissues in a plant and hence was not expressed at the cellular level. |
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