Plant regeneration from protoplasts of the monocotyledonous Haworthia magnifica v. Poelln. |
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| Authors: | Yongru Sun Berthold M Heil Günter Kahl Hans Willy Kohlenbach |
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| Institution: | (1) Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstraße 70, D-6000 Frankfurt am Main, FRG;(2) Present address: Institute of Genetics, Academia Sinica, Beijing |
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| Abstract: | Calli were initiated from flower buds, gynoecia and inflorescence segments of Haworthia magnifica v. Poelln. and subcultured on solid medium. Two liquid culture steps were necessary to prepare the calli for the isolation of protoplasts capable of sustained cell divisions. Plants were regenerated from protoplast-derived calli. The influence of both the osmolality of the culture media and exudates on the viability of protoplasts and protoplast-derived cell colonies is briefly discussed. |
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| Keywords: | protoplast culture plant regeneration monocotyledons Haworthia magnifica v Poelln |
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