Utility of HBME-1 immunostaining in serous effusions |
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Authors: | V ASCOLI C CARNOVALE-SCALZO S TACCOGNA F NARDI |
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Institution: | Laboratorio di Citodiagnostica, Dipartimento di Medicina Sperimentale e Patologia, Università'LA Sapienza', Roma, Italy |
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Abstract: | Utility of HBME-1 immunostaining in serous effusions HBME-1 is an anti-mesothelial cell monoclonal antibody derived from human mesothelioma cells. We investigated 227 body cavity effusions to test its utility in differentiating mesothelioma from adenocarcinoma. HBME-1 outlined cell membranes in non-neoplastic mesothelial cells. Thick surface staining was observed on all mesotheliomas. HBME-1 reactivity was also detected in 24% of metastatic carcinomatous effusions. Most ovarian carcinomas (83%) reacted with this antibody, showing surface staining. Cytoplasmic HBME-1 immunoreactivity was observed in a small proportion of non-ovarian adenocarcinomas (14%). Despite its limited specificity, HBME-1 might be added to the battery of other markers of epithelial and/or mesothelial differentiation to be used in cases of suspected mesothelioma. Evaluation of suspicious cells should include careful study of the pattern of immunostaining. |
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Keywords: | cancer-related antigens immunocytochemistry adenocarcinoma mesothelioma mesothelial cells cytodiagnosis effusion |
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