Phage antibody display libraries: a powerful antibody discovery platform for immunotherapy |
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| Authors: | Aizhi Zhao Donald L Siegel George Coukos |
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| Institution: | 1. Ovarian Cancer Research Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA,;2. Division of Transfusion Medicine, Department of Pathology &3. Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA, and;4. Ovarian Cancer Research Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA,;5. Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland |
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| Abstract: | Phage display technology (PDT), a combinatorial screening approach, provides a molecular diversity tool for creating libraries of peptides/proteins and discovery of new recombinant therapeutics. Expression of proteins such as monoclonal antibodies (mAbs) on the surface of filamentous phage can permit the selection of high affinity and specificity therapeutic mAbs against virtually any target antigen. Using a number of diverse selection platforms (e.g. solid phase, solution phase, whole cell and in vivo biopannings), phage antibody libraries (PALs) from the start point provides great potential for the isolation of functional mAb fragments with diagnostic and/or therapeutic purposes. Given the pivotal role of PDT in the discovery of novel therapeutic/diagnostic mAbs, in the current review, we provide an overview on PALs and discuss their impact in the advancement of engineered mAbs. |
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| Keywords: | Cancer immunotherapy phage antibody library phage display technology recombinant monoclonal antibody |
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