Isolation and production of artemisinin and stigmasterol in hairy root cultures of Artemisia annua |
| |
Authors: | Xie Deyu Wang Lianhui Ye Hechun Li Guofeng |
| |
Institution: | (1) Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China;(2) Institute of Polymer Science, Fujian Teachers University, Fuzhou, 350007, People's Republic of China |
| |
Abstract: | Scaled-up hairy root culture of Artemisia annua L. was established in three-liter Erlenmeyer flask. Both artemisinin and stigmasterol that derive from the common precursors
of isopentenyl diphosphate and farnesyl pyrophosphate were isolated from hairy roots. The production rate of artemisinin isolated
by column chromatography from hairy root cultures was 0.54% (mg.gDW−1). Stigmasterol was identified by mass spectrometry and nuclear magnetic resonance analysis. The production of stigmasterol
isolated by column chromatography from hairy root cultures was 108.3% (mg.gDW−1). In hairy root cultures, the production rate of stigmasterol was estimated to be 201 times greater than that of artemisinin.
Our results suggest that investigation of secondary metabolites may provide a new insight to study artemisinin production
in hairy root cultures.
This revised version was published online in August 2006 with corrections to the Cover Date. |
| |
Keywords: | Artemisia annua artemisinin hairy root culture stigmasterol |
本文献已被 SpringerLink 等数据库收录! |