枯草芽孢杆菌BF7658噬菌体的研究

从一些工厂分离到6株感染枯草芽孢杆菌BF7658的噬菌体,对它们的形态学和生物学特性以及DNA和结构蛋白进行了比较,电镜观察指出,所有噬菌体的头部外形都呈现椭园形,但其大小和尾部长度有所不同,它们的宿主范围较窄,用限制酶分析,噬菌体DNA分子量在29.9kb和98kb之间,根据解链温度计算出噬菌体DNA的G+C含量在45.1和60.2mol％之间,6株噬菌体的蛋白经SDS-聚丙烯酰胺凝胶电泳测定呈现四条主带和五条次带。

PROPERTIES OF SIX BACILLUS SUBTILIS BF7658 BACTERIOPHAGES

Six lytic bacteriophages were isolated from samples of Bacillus subtilis BF7658.The morphology, some biological properties of the phages and the physicochemical characteristics of their DNAs were compared. Electron-microscopic observation indicated that all phage heads appeared to be elongated, but the head size and the length of the tails varied. These phages had a narrow host range. The molecular weights of the phage DNAs were between 29.9 and 98 kb by EcoRI enzyme analysis. The G + C content of the DNAs ranged between 45.1 and 60.2 mol% as estimated from melting temperture. All six strains gave four major protein bands and at least five minor ones as analysed by SDS-polyacrylamide gel electrophoresis.