Overexpression of Jatropha curcas Defensin (JcDef) Enhances Sheath Blight Disease Resistance in Tobacco |
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| Authors: | Qingwei Wang Shuhua Zhu Yao Liu Rui Li Shancai Tan Shenghua Wang Lin Tang Fang Chen |
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| Institution: | 1. Key Lab of Bio‐Resources and Eco‐Environment, Ministry of Education, College of Life Science, Chengdu, China;2. National and Local Joint Engineering Laboratory for Energy Plant Bio‐Oil Production and Application, Sichuan University, Chengdu, China;3. Rice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, China |
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| Abstract: | Plant defensins are small, basic, cysteine‐rich peptides, belonging to the antimicrobial peptide superfamily, commonly found in the plant kingdom. In this study, we cloned and characterized a plant defensin gene from Jatropha curcas (JcDef). JcDef carried conserved receptor binding sites and a cysteine motif, and it was phylogenetically grouped together with defensin Ec‐AMP‐D2‐like in Elaeis guineensis. JcDef is localized to cytoplasm and highly expressed in young tissues with fast metabolism such as cotyledons and stem apexes. Transgenic expression of JcDef in tobacco showed enhanced resistance against sheath blight disease caused by R. solani, indicating the antibacterial function. |
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| Keywords: | defensin disease resistance
Jatropha curcas
sheath blight transgenic |
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