The regulation of Mn2+ and Cu2+ uptake in cells of the yeast Candida utilis grown in continuous culture |
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Authors: | Michael J Parkin IStuart Ross |
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Institution: | Department of Biological Sciences, University of Keele, Keele, Staffordshire, ST5 5BG, U.K. |
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Abstract: | Abstract Transport of Mn2+ was repressed in Candida utilis cells grown in continuous culture in high-Mn2+ (100 μM Mn2+) medium as compared to cells grown in basic (0.45 μM Mn2+) and low-Mn2+ (< 0.05 μM Mn2+) media. In contrast, no repression of Cu2+ uptake occurred in high-Cu2+-grown (25 μM Cu2+) cells as compared to cells grown in basic medium (0.54 μM Cu2+). Cu2+-limited cells did not hyperaccumulate Cu2+ and there was not significant difference in initial uptake rates for all 3 Cu2+ conditions. Mn2+ uptake appears to be regulated by a mechanism sensitive to the external Mn2+ concentration, whereas Cu2+ transport is not governed in this way by the external Cu2+. |
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Keywords: | Candida utilis manganese transport copper transport chemostat |
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