|
|||||
|
|
| 偏分离分子标记的作图方法 | |
| 引用本文： | 谭军,薛庆中. 偏分离分子标记的作图方法[J]. 遗传, 2004, 26(3): 356-360 |
| 作者姓名： | 谭军 薛庆中 |
| 作者单位： | 浙江大学农业与生物技术学院农学系,杭州,310029 |
| 基金项目： | 国家863计划(101-01-01-01-3)，浙江省重点科研项目(2003C22007)~~ |
| 摘 要： | 对取自MAPMAKER软件小鼠F_2群体(含333个体)的5个RFLP连锁标记数据作了共显性分子标记偏分离的分析。先确定选择类型的方程组(配子或合子),随后采用Newton-Raphson迭代法估算标记间的重组值。在构建分子标记遗传图谱时,如果两个相邻标记均存在偏分离,最好采用纳入偏分离因子的估算方法。在估计F_2群体标记间偏分离重组距离上,用连续x~2检测方法比传统x~2检测更为准确。 |
| 关 键 词： | 分子标记 遗传作图 偏分离 |
| 文章编号： | 0253-9772(2004)03-0356-05 |
| 修稿时间： | 2003-03-21 |
Mapping by Molecular Markers Showing Segregation Distortion |
|
| TAN Jun,XUE Qing-Zhong. Mapping by Molecular Markers Showing Segregation Distortion[J]. Hereditas, 2004, 26(3): 356-360 | |
| Authors: | TAN Jun XUE Qing-Zhong |
| Affiliation: | Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China. |
| Abstract: | The comparative analysis of segregation distortions of the codominant markers data presented in softwareMAPMAKER are made, where five RFLPs markers involve in a mouse F_2 Population with 333 individuals. The suc-cessive x~2 test begins with the determinations of gametic or zygotic selection types, followed by the estimation of re-combination fractions between two markers with the Newton-Raphson iteration method. It is better to use the mo-lecular marker showing segregation distortion for constructing a genetic map, in the case of seriously skew segrega-tion between both the adjoining markers. The successive x~2 test provides better accuracy than that of classical x~2 testfor the estimation of the recombination values in F_2 population with segregation distortion. |
| Keywords: | molecular marker genetic mapping segregation distortion |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |