| 在小鼠胚胎干细胞进行基因打靶的策略 |
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| 引用本文： | 杨晓,张兆山,黄翠芬.在小鼠胚胎干细胞进行基因打靶的策略[J].生物化学与生物物理进展,1997,24(2):104-108. |
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| 作者姓名： | 杨晓 张兆山 黄翠芬 |
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| 作者单位： | 军事医学科学院生物工程研究所，北京 100071;军事医学科学院生物工程研究所，北京 100071;军事医学科学院生物工程研究所，北京 100071 |
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| 摘 要： | 基因打靶技术是一种通过同源重组按预期方式改变生物活体的遗传信息的实验手段，与小鼠胚胎干细胞培养系统相结合，使得人们可以方便地将各种突变引入小鼠体内，得以从生物整体水平上研究高等真核生物基因的表达、调控及其生理功能.扼要介绍了近年来在小鼠胚胎干细胞进行基因打靶的研究进展.
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| 关 键 词： | 基因打靶 小鼠胚胎干细胞 同源重组 打靶载体 |
| 收稿时间： | 4/9/1996 12:00:00 AM |
| 修稿时间： | 1996/9/23 0:00:00 |
Strategies of Gene Targeting in Mouse Embryonic Stem Cell |
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| YANG Xiao,ZHANG Zhaoshan and HUANG Cuifen.Strategies of Gene Targeting in Mouse Embryonic Stem Cell[J].Progress In Biochemistry and Biophysics,1997,24(2):104-108. |
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| Authors: | YANG Xiao ZHANG Zhaoshan and HUANG Cuifen |
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| Institution: | Institute of Biotechnology, Academy of Millitary Medical Sciences, Beijing 100071,China;Institute of Biotechnology, Academy of Millitary Medical Sciences, Beijing 100071,China;Institute of Biotechnology, Academy of Millitary Medical Sciences, Beijing 100071,China |
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| Abstract: | Gene targeting is an experimental technique which could alter the genetic message of lives following the anticipated pattern through homologous recombination. Gene targeting combined with the system of the culture of the mouse embryonic stem cell facilitate introducing various mutations into living mouse, so that the expression, regulation and physiological function of higher eukaryotic genes could be studied as a whole. Recent progress in studying on gene targeting in mouse embryonic stem cell was introduced briefly. |
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| Keywords: | gene targeting mouse embryonic stem cells homologous recombination targeting construct |
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