| 轮状病毒LH9株在Vero细胞中的培养条件优化研究 |
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| 引用本文： | 包红,寇桂英,王名强,韩平,余黎,周旭.轮状病毒LH9株在Vero细胞中的培养条件优化研究[J].微生物学免疫学进展,2011,39(1):15-19. |
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| 作者姓名： | 包红 寇桂英 王名强 韩平 余黎 周旭 |
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| 作者单位： | 兰州生物制品研究所,兰州,730046 |
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| 摘 要： | 为优化轮状病毒株在Vero细胞上的培养条件,将轮状病毒基因重配株LH9按0.1MOI分别接种于不同规格的细胞培养瓶(100ml、2000ml、3L、15L)。病毒接种采用吸附与未吸附两种方式、病毒收获采取低温冻融后离心与直接离心两种方法,观察分析对病毒滴度的影响。实验中,用CASY细胞计数仪分析活细胞率,病毒接种后逐日观察细胞病变(CPE)并取样,采用细胞半数感染量测定病毒滴度。结果表明,使用不同规格细胞培养瓶经吸附法培养接种、低温破碎法收获的LH9株病毒滴度高,其中以15L立瓶培养滴度最高(6.0～7.0 logCCID50/ml)。
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| 关 键 词： | 轮状病毒 基因重配株 Vero细胞 高滴度 |
Culture conditions optimization of rotavirus LH9 on Vero cells |
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| BAO Hong,KOU Gui-ying,WANG Ming-qiang,et al..Culture conditions optimization of rotavirus LH9 on Vero cells[J].Progress In Microbiology and Immunology,2011,39(1):15-19. |
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| Authors: | BAO Hong KOU Gui-ying WANG Ming-qiang |
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| Institution: | BAO Hong,KOU Gui-ying,WANG Ming-qiang,et al.(Lanzhou Institute of Biological Products,Lanzhou,730046,China) |
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| Abstract: | In order to optimize the culture conditions of rotavirus strains on Vero cells,the strain LH9 was inoculated in cell culture bottles with different volume(100ml,2000ml,3L,15L)by 0.1 MOI.The two inoculation methods(adsorption or non-adsorpttion) and two harvesting methods(direct centrifugalizing and centrifugalizing after freeze-thaw) were adopted to observe the effects of these methods on the virus titers.The viability of the cell was analyzed using the CASY Cell Counter,the CPE of Vero cells after inoculat... |
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| Keywords: | Rotavirus Reassortant Vero cells High titers |
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