The effect of short-term H2S fumigation on water-soluble sulphydryl and glutathione levels in spinach |
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Authors: | L J DE KOK W BOSMA F M MAAS P J C KUIPER |
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Institution: | Department of Plant Physiology, University of Groningen, P.O. Box 14, 9750 AA Haren (Gn), The Netherlands |
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Abstract: | Abstract. Short-term fumigation of Spinacia oleracea with 380 μg m?3 H2S (250 ppb) resulted in a rapid accumulation of water-soluble SH-compounds in the shoots. After 1 h exposure a substantial increase in the SH-content was already detectable and maximal accumulation, three- to four-fold that in control plants, was observed after 24 h of exposure. Irradiation during H2S exposure only slightly affected the rate and level of SH-accumulation. H2S fumigation did not affect the water-soluble SH-content of the roots. Glutathione was the sole water-soluble SH-compound accumulating upon exposure to H2S. It was calculated that during the first hour of exposure to 380 μg m?3 H2S 39% of the possible absorbed H2S was converted into glutathione. The SH-content of the water-soluble proteins of the shoots was not affected by H2S exposure. When fumigation was stopped, a rapid decrease in glutathione content was observed and after 48 h the content was comparable to that of the control plants. Contrary to H2S, SO2 fumigation did not result in a rapid accumulation of glutathione in spinach shoots. The possible role of glutathione accumulation during H2S fumigation is discussed. |
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Keywords: | Spinacia oleracea hydrogen sulphide sulphur dioxide glutathione total protein sulphydryl |
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