不同地区致倦库蚊种群相关酯酶 基因的特征分析

分别从广州、沙市、武汉近郊采集到致倦库蚊Culex pipiens quinquefasciatus，对单只蚊虫进行淀粉凝胶电泳和Southern杂交方法的分析结果表明：3个实验种群中均分布有与抗性有关的高活性酯酶β1¹，酯酶α2/β2分布于广州实验种群中；广泛分布于地中海地区尖音库蚊Culex pipiens种群中的酯酶α4/β4、α5/β5在以上3个种群中均不存在。但是，在3个实验种群中均发现存在有一对新的高活性酯酶α8/β8，其电泳迁移率和限制性酶切片段均与目前已报道的几种高活性酯酶不同。含这两对新酯酶的蚊虫将应进一步从种群中纯化，纯合蚊虫新品系做分子特征的研究。

Characterizations of related esterase genes in Culex pipiens quinquefasciatus from different geographical regions

Culex pipiens quinquefasciatus sampled in Guangzhou in China, has larval resistance to organophosphate insecticides. The results of starch gel electrophoresis and Southern hybridization indicate that there are seven highly active esterases:esterase β11，α2/β2，α6/β6 and α8/β8 in this population. A new esterase haplotype(esterase α8/β8) was found in the populations. The results of the migration rate and RFLP polymorphism of these highly active esterases indicate the esterase α8/β8 is different from the others. In the same way,Culex pipiens quinquefasciatus populations sampled from Wuhan and Shashi in China, were analyzed. Also a new pair of esterase α8/β8 are involved in Wuhan and Shashi populations.