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Production of waxy (amylose-free) wheats
Authors:Toshiki Nakamura  Makoto Yamamori  Hisashi Hirano  Soh Hidaka and Tukasa Nagamine
Institution:(1) Tohoku National Agricultural Experiment Station, Akahira 4, 020-01 Morioka, Iwate, Japan;(2) Japan International Research Center for Agricultural Sciences, Okinawa Branch, 907 Ishigaki, Okinawa, Japan;(3) National Institute of Agrobiological Resources, Kannondai 2-12, 305 Tsukuba, Ibaraki, Japan
Abstract:The Waxy (Wx) protein has been identified as granule-bound starch synthase (GBSS; EC 24.1.21), which is involved in amylose synthesis in plants. Although common wheat (Triticum aestivum L.) has three Wx proteins, ldquopartial waxy mutantsrdquo lacking one or two of the three proteins have been found. Using such partial waxy mutants, tetra- and hexaploid waxy mutants with endosperms that are stained red-brown by iodine were produced. Both mutants showed loss of Wx protein and amylose. This is the first demonstration of genetic modification of wheat starch.
Keywords:Wheat  Waxy  Amylose  Wx protein  Mutant
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