Determination of reduced glutathione in guinea pig and rat tissue by HPLC with electrochemical detection |
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| Authors: | Ivan Mefford Ralph N. Adams |
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| Affiliation: | Department of Chemistry University of Kansas, Lawrence, Kansas 66045, USA |
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| Abstract: | A method using HPLC with electrochemical detection has been developed for the determination of GSH in tissue. The method is based upon the separation of GSH from other components by cation exchange chromatography coupled with the electrochemical oxidation of GSH to the corresponding disulfide. Detection limits of ca. 5 × 10?12 moles GSH were established and the method was used to measure GSH content of rat and guinea pig brain, liver and synaptosome preparations. |
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