Lack of dependence of amine or prostaglandin biosynthesis on absolute cerebral level of pteridine cofactor |
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Authors: | AD Sherman EM Gál |
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Institution: | Neurochemical Research Laboratories Department of Psychiatry College of Medicine University of Iowa 500 Newton Road Iowa City, Iowa 52242, USA |
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Abstract: | Using 2-amino-6-(5'-2'-deoxyphosphoribosyl)-amino-5- or -6-formamido-6-hydroxypyrimidine (dFPyd-P3), a specific inhibitor of tetrahydrobiopterin (BH4) synthesis, cerebral pools of BH4 were reduced to half of that of controls; while, simultaneously, the biosynthesis of L-erythrodihydroniopterin (BH2) from GTP was inhibited by about 98%. Nevertheless, there was no effect on the cerebral levels of serotonin, dopamine, norepinephrine or on the biosynthesis of prostaglandin E2 or F1. The data are presented in evidence that the absolute level of the cofactor (BH4) is not regulatory of amine or protaglandin biosynthesis . Amine and prostaglandin biosynthesis proceeded even at cofactor concentrations of 9×107 M nsuggesting that their biosynthesis is dependent on the rate of H+ + e shuttle between BH2 and BH4. |
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