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耐高温木质素降解菌筛选及降解效果研究
引用本文:郭玲玲,江志阳,陶姝宇,于淼,朱晓琳,陈丽媛.耐高温木质素降解菌筛选及降解效果研究[J].微生物学杂志,2022(4):64-69.
作者姓名:郭玲玲  江志阳  陶姝宇  于淼  朱晓琳  陈丽媛
作者单位:辽宁省微生物科学研究院,辽宁 朝阳 122000;中国科学院 沈阳应用生态研究所,辽宁 沈阳 110016;辽宁省农业发展服务中心,辽宁 沈阳 110032;辽宁恒润农业有限公司,辽宁 鞍山 114000
基金项目:辽宁省凌源市食用菌科技特派团项目(2021JH5/10400065);辽宁省凌源市食用菌乡村振兴科技特派团项目(2021JH5/10400098);辽宁省科技重大专项(2020JH1/10300006);吉林省与中科院专项资金项目(2020SYHZ0044);国家重点研发计划项目(2019YFD1100504)
摘    要:为提高育苗基质中废弃物木质素降解速率,在废弃物堆腐生产育苗基质高温阶段取样,筛选耐高温木质素降解菌,并对菌种进行鉴定,同时测定其对秸秆木质素和菌糠木质素的降解效果。获得了1株较好的木质素高温降解菌HZ11,鉴定为解淀粉芽胞杆菌(Bacillus amyloliquefaciens),结果显示,该菌株对秸秆木质素和菌糠木质素降解效果较好,50 ℃条件下,20 d木质素降解率分别为46.7%和42.4%。菌株HZ11在降解秸秆和菌糠方面具有很好的应用潜力,为利用农业废弃物生产育苗基质提供更加丰富的菌种资源,具有重要的参考价值。

关 键 词:解淀粉芽胞杆菌  耐高温  木质素  降解效果  菌种鉴定

Screening and Degradation Effect of High Temperature Resistant Lignin Degradable Bacteria
GUO Ling-ling,JIANG Zhi-yang,TAO Shu-yu,YU Miao,ZHU Xiao-lin,CHEN Li-yuan.Screening and Degradation Effect of High Temperature Resistant Lignin Degradable Bacteria[J].Journal of Microbiology,2022(4):64-69.
Authors:GUO Ling-ling  JIANG Zhi-yang  TAO Shu-yu  YU Miao  ZHU Xiao-lin  CHEN Li-yuan
Institution:Liaoning Acad. of Microbiol. Chaoyang 122000;Shenyang Inst. of Appl. Ecol., China Acad. of Sci., Shenyang 110016;Liaoning Prov. Agric. Devel. Serv. Ctr., Shenyang 110032;Liaoning Hengrun Agric. Co. LTD, Anshan 114000
Abstract:The aim of this study was to screen high temperature resistant lignin degradable bacteria. In this study, the agricultural biomass waste in seedling substrate was used as raw material to screen and identify the lignin degradable strains, and the degradation effects of the strains on straw lignin and bran lignin were measured. The results showed that a good lignin degradable strain HZ11 was obtained and identified as Bacillus amyloliquefaciens. It had a good degradation effect on straw lignin and bran lignin, and the lignin degradation rates were 46.7% and 42.4% within 20 d. Strain HZ11 has a good application potential, and it is worthy of further research in the future, so as to provide more abundant strain resources and theoretical basis for the resource utilization of agricultural waste.
Keywords:Bacillus amyloliquefaciens  high temperature resistant  lignin  degradation effect  strain seed identification
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