白背飞虱若虫和雄雌成虫唾液腺蛋白鉴定

【目的】对白背飞虱Sogatella furcifera唾液腺蛋白进行鉴定及功能注释分类，探究不同发育阶段和不同性别的白背飞虱唾液腺蛋白之间的区别与联系。【方法】麻醉白背飞虱若虫、雄成虫和雌成虫，解剖收集唾液腺组织，提取蛋白，还原烷基化和酶解，利用液相色谱串联质谱技术鉴定蛋白。与Unigene蛋白数据库进行比对，并通过KOG分析，对唾液腺蛋白进行功能注释分类。【结果】白背飞虱若虫、雄成虫和雌成虫特有的唾液腺蛋白分别为385, 168和82个，若虫与雄成虫、若虫与雌成虫及雄成虫与雌成虫共有的唾液腺蛋白分别为319, 60和60个。KOG功能注释显示与细胞过程和信号传导相关的蛋白数量最多，若虫、雄成虫和雌成虫特有以及若虫与雄成虫、若虫与雌成虫及雄成虫与雌成虫共有的这类蛋白数量分别为81, 22, 70, 19, 21和12个，这些蛋白主要发挥着翻译后修饰、蛋白质更新、伴侣蛋白，细胞内运输、分泌和囊泡运输及信号转导作用。【结论】白背飞虱唾液腺蛋白在参与信号转导机制方面表现较为活跃，这可能与其刺吸危害有一定的关系。

Identification of salivary gland proteins of nymphs and male and female adults of Sogatella furcifera (Hemiptera: Delphacidae)

【Aim】 To identify and functionally annotate the salivary gland proteins ofSogatella furcifera and to explore their differences and correlation between different developmental stages and sexes. 【Methods】 Proteins were extracted from salivary gland tissues dissected from anesthetized nymphs, and male and female adults of S. furcifera, subjected to reductive alkylation and enzymolysis, and identified by liquid chromatography tandem mass spectrometry. Then, the salivary gland proteins were functionally annotated and classified by comparison with the Unigene protein database and KOG analysis. 【Results】There were 385, 168 and 82 salivary gland proteins specifically associated with nymphs, and male and female adults of S. furcifera, respectively. In total 319 salivary gland proteins were shared by nymphs and male adults, 60 by nymphs and female adults, and 60 by male and female adults. The KOG functional annotation showed the highest number of proteins associated with cellular processes and signaling, with 81, 22, and 70 proteins of this type specifically associated with nymphs, male adults, and female adults, respectively, and 19, 21 and 12 proteins of this type shared by nymphs and male adults, nymphs and female adults, and male and female adults， respectively, and these proteins are principally involved in post-translational modification, protein turnover, chaperones, intracellular trafficking, secretion and vesicular transport, and signal transduction.【Conclusion】 The salivary gland proteins of S. furcifera are quite active in signal transduction, which may be connected to its piercing and sucking damage to host plants.