How bryophytes came out of the cold: successful cryopreservation of threatened species |
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Authors: | J K Rowntree M M Ramsay |
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Institution: | (1) Micropropagation Unit, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, UK;(2) Present address: Faculty of Life Sciences, University of Manchester, Michael Smith Building, Manchester, M13 9PT, UK |
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Abstract: | The use of ex situ techniques for the conservation of threatened plants has been increasing over the past decades. Cryopreservation
is often used for the long-term storage of plant germplasm where conventional methods (i.e. seedbanking) are inappropriate.
Simple encapsulation–dehydration protocols were developed for the cryopreservation of bryophytes at The Royal Botanic Gardens,
Kew, as part of an ex situ project for the conservation of UK threatened species. The applicability of these methods was tested
on 22 species with a broad range of ecological requirements and found to be highly successful across the board. Regeneration
rates from frozen material were >68% for all species tested and half had regeneration rates of 100%. The high regeneration
rate and broad applicability of the protocols across a range of species was attributed to a combination of the inherent totipotency
of bryophytes and the in-built recovery periods in the pre-treatment protocol. In conclusion, bryophytes are well suited to
cryopreservation and such techniques would be applicable for the long-term storage of similar conservation collections across
the globe. |
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Keywords: | Bryophyte Cryopreservation Encapsulation– dehydration Ex situ conservation Threatened species |
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