Electric field-mediated cell fusion |
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Authors: | U. Zimmermann G. Pilwat H. A. Pohl |
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Affiliation: | (1) Arbeitsgruppe Membranforschung am Institut fürMedizin, Kernforschungsanlage Jülich, Postfach 1913, D-5170 Jülich, West Germany;(2) Department of Physics, Oklahoma State University, 74078 Stillwater, OK, USA |
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Abstract: | The use of electrical fields to guide, hold and fuse cells is described. The electrical fusion process consists of two steps: the cells are collected to form pearl-chains between Pt electrodes by the action of dielectrophoresis, then a brief DC pulse is applied, such that the breakdown voltage of the membranes is briefly exceeded and cell-to-cell juncture of the membranes occurs around the pore formed by the pulse. Giant fused cells (diameter up to 100 m) can be formed by the electrically mediated fusion of mouse 3T3 fibroblast cells, provided that pronase is added just before field application. |
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