Determination of opipramol in human plasma by high-performance liquid chromatography with photometric detection using a cyanopropyl column |
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| Authors: | F Kees M Bucher G Mair H Grobecker |
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| Institution: | a Department of Pharmacology, University of Regensburg, Universitätsstraβe 31, D-93053 Regensburg, Germany;b Department of Anesthesiology, University of Regensburg, D-93053 Regensburg, Germany |
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| Abstract: | A high-performance liquid chromatographic method is described for the determination of opipramol in human plasma. Opipramol was extracted into tert.-butylmethyl ether, separated on a cyanopropyl silica column and detected at 254 nm. Imipramine was used as internal standard. The limit of quantitation was 250 pg/ml using 1.5 ml plasma. Precision was better than 9%, inaccuracy less than 8%. The assay is more sensitive than previously published methods, and it has been applied to the analysis of plasma samples from a pharmacokinetic study. |
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| Keywords: | Opipramol |
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