Abstract: | Photosynthesis under conditions known to favour glycollate excretionby algae did not result in glycollate excretion in a strainof Chlorella pyrenoidosa unless an inhibitor of glycollate oxidase,-hydroxypyridin-2yl-methane sulphonate (-HPMS), was present.This inhibitor increased the total amount of glycollate presentin the supernatant from the cells during photosynthetic carbondioxide fixation and gave accumulation of 14C in glycollateduring 14CO2 fixation under conditions favouring glycollatesynthesis. At pH 8.3 -HPMS did not stimulate photosynthetic14CO2 fixation in C. pyrenoidosa as occurs with some algae.Photoassimilation of acetate was inhibited by -HPMS, and thiswas shown to result from acetyl-CoA synthetase inhibition by-HPMS. |