食蟹猴血液学及血清生化指标日间节律性变化

目的研究食蟹猴血液学及血清生化指标日间节律性变化,为正确全面评估血液学及血清生化测定结果提供一定的实验事实依据。方法在9：30（am）～10：30（am）、12：30（pm）～13：30（pm）、15：30（pm）～16：30（pm）及19：30（pm）～20：30（pm）4个时间段对12只食蟹猴（雌雄各半）采血。采用全自动血细胞分析仪及血液生化分析仪分别测定血液学及血清生化指标。利用高效液相色谱（HPLC）方法分析血浆中皮质醇含量。结果雌雄食蟹猴下午白细胞数（WBC）及尿素氮（BUN）显著升高;雌性食蟹猴下午血红蛋白（HGB）及血细胞压积（HCT）显著降低,天门冬氨酸氨基转换酶（AST）及丙氨酸氨基转换酶（ALT）活性显著升高;雄性食蟹猴下午血糖（GLU）及血浆皮质醇显著降低。结论雌雄食蟹猴血液学及血清生化部分指标存在明显的日间节律性。

Diurnal Variations of Hematological and Biochemical Parameters in Cynomolgus Monkeys （Macaca fascicularis）

Objective To investigate the diurnal variations of hematological and biochemical parameters in cynomolgus monkeys.Methods The blood samples were collected in 12 cynomolgus monkeys（6 of each sex） at 9：30（am） ～ 10：30（am）,12：30（pm） ～ 13：30（pm）,15：30（pm） ～ 16：30（pm） and 19：30（pm） ～ 20：30（pm）.The hematological and biochemical parameters were determined with automated hematological and biochemical analyzers respectively.The plasma cortisol levels were determined by high-performance liquid chromatography（HPLC）.Results White blood count（WBC） and blood urea nitrogen（BUN） increased significantly in the afternoon in male and female monkeys.Hemoglobin（HGB） and hematocrit（HCT） markedly reduced in the afternoon in female monkeys.The activities of asparate aminotransferase（AST） and alanine aminotransferase（ALT） significantly increase in the afternnon in female monkeys.The serum glucose and plasma cortisol reduced significantly in the afternoon in male monkeys.Conclusions Parts of the hematological and biochemical parameters show obvious diurnal variation in male and female cynomolgus monkeys.