| N-甲基亚硝基脲诱导小鼠胸腺淋巴瘤TCR基因重排分析 |
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| 引用本文： | 黄榕芳,余英豪,李博.N-甲基亚硝基脲诱导小鼠胸腺淋巴瘤TCR基因重排分析[J].中国实验动物学杂志,2010(9):7-11. |
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| 作者姓名： | 黄榕芳 余英豪 李博 |
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| 作者单位： | [1]南京军区福州总医院病理科,福州350025 [2]南京军区福州总医院实验科,福州350025 |
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| 基金项目： | 南京军区医学科学技术研究“十一五”计划课题资助（06MA-147 07Z034）; 福建省自然科学基金（2010J01221） |
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| 摘 要： | 目的探讨N-甲基亚硝基脲（MNU）诱导的小鼠胸腺淋巴瘤的单克隆起源。方法采用巢式PCR方法,对8例MNU诱导的胸腺淋巴瘤组织进行T细胞受体β链（TCRβ）和γ链（TCRγ）克隆性基因重排分析,并对TCRγ基因重排的PCR产物直接测序。结果 8例胸腺淋巴瘤检测TCRβ和TCRγ均呈克隆性基因重排。DNA序列测定证实TCRγ基因PCR扩增产物为基因重排产物。结论巢式PCR TCR基因重排检测及DNA序列分析证实,MNU诱导的小鼠胸腺淋巴瘤是来源于T细胞的肿瘤。
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| 关 键 词： | 恶性淋巴瘤 胸腺 N-甲基亚硝基脲/MNU T细胞受体 聚合酶链反应 基因重排 |
The Analysis of TCR Gene Rearrangements in N-methyl-N-nitrosourea（MNU）-induced Thymic Lymphoma in Mice |
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| HUANG Rong-fang,YU Ying-hao,LI Bo.The Analysis of TCR Gene Rearrangements in N-methyl-N-nitrosourea（MNU）-induced Thymic Lymphoma in Mice[J].Chinese Journal of Laboratory Animal Science,2010(9):7-11. |
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| Authors: | HUANG Rong-fang YU Ying-hao LI Bo |
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| Institution: | 1.Department of Pathology,Fuzhou General Hospital,Nanjing Military Command,Fuzhou 350025,China;2.PLA Center for Laboratory Medicine,Fuzhou General Hospital,Nanjing Military Command,Fuzhou 350025,China) |
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| Abstract: | Objective To detect the monoclonal origin of N-methyl-N-nitrosourea（MNU）-induced thymic lymphoma in mice.Methods 8 samples of MNU-induced thymic lymphoma were analyzed with nested polymerase chain reaction（PCR） for TCRβ and TCRγ clonal gene rearrangements.The PCR products of TCRγ gene rearrangements were purified and directly sequenced.Results 8 cases of thymic lymohomas were all detected TCRβ and TCRγ clonal gene rearrangements.DNA sequence analysis confirmed that the PCR products generated were indeed TCRγ rearrangements.Conclusion Just as determined by TCR gene rearrangements analysis and DNA sequence analysis,the MNU-induced thymic lymphoma in mice is a T-cell origin. |
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| Keywords: | Malignant lymphoma Thymus N-methyl-N-nitrosourea/MNU T-cell receptor Polymerase chain reaction Gene rearrangement |
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