| 人脑神经元特异性烯醇化酶的纯化方法 |
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| 引用本文： | 郭健,张世明,周润琦,陈石根,韩一平.人脑神经元特异性烯醇化酶的纯化方法[J].生物化学与生物物理进展,1995,22(6):552-555. |
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| 作者姓名： | 郭健 张世明 周润琦 陈石根 韩一平 |
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| 作者单位： | 江苏常州102医院内科;长海医院呼吸内科;复旦大学生物化学系;复旦大学生物化学系;长海医院呼吸内科 |
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| 基金项目： | 国家自然科学基金资助项目. |
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| 摘 要： | 采用改良的Grace层析方法,经一次DEAE-Sephadex A50柱层析即从人脑中纯化了神经元特异性烯醇化酶,比活力为92.1U/mg,纯化倍数为59.4.该酶纯化后,经SDS-聚丙烯酰胺凝胶电泳鉴定为单一蛋白质谱带.此外,还测定了其部分理化性质,其亚单位分子量为45000,等电点pI为4.7,氨基酸组成分析表明其为一种酸性蛋白质;对2-磷酸甘油酸的Km值为5.6×10-4mol/L.
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| 关 键 词： | 神经元特异性烯醇化酶 纯化 理化性质 |
| 收稿时间： | 1994/12/24 0:00:00 |
| 修稿时间： | 1995/2/26 0:00:00 |
A Convenient Method for the Purification of Neuron Specific Enolase |
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| Guo Jian,Zhang Shiming,Zhou Runqi,Chen Shigen and Han Yipin.A Convenient Method for the Purification of Neuron Specific Enolase[J].Progress In Biochemistry and Biophysics,1995,22(6):552-555. |
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| Authors: | Guo Jian Zhang Shiming Zhou Runqi Chen Shigen and Han Yipin |
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| Institution: | Department of Respiratory; Chang Hai Hospital;Department of Respiratory; Chang Hai Hospital;Department of Biochemistry; Fudan University;Department of Biochemistry; Fudan University;Department of Respiratory; Chang Hai Hospital |
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| Abstract: | A simple and convenient method for the purification of neuron specific enolase (NSE)from human brain is described through only one DEAE-Sephadex A50 chromatography.The specific activity is 92.1U/mg and increase of purity 59.4 fold.Certain biophysical and biochemical features were also studied.The molecular weight of the subunit of NSE is 45 000 and pI is 4.7.Amino acid composition analysis showed that NSE is an acidic protein. |
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| Keywords: | neuron specific enolase purification biophysical and biochemical features |
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