南方红豆杉和东北红豆杉中的单体化合物对乳腺癌细胞增殖的影响

本研究采用MTT法,研究南方红豆杉和东北红豆杉中的10种不同结构类型的单体化合物对乳腺癌MCF-7细胞增殖的影响。结果表明,化合物1~10(10-9~10-5mol/L)处理MCF-7细胞48和72 h后,仅化合物4在10-7、10-6和10-5mol/L浓度对MCF-7细胞增殖均有明显抑制作用,抑制率分别为29.8%、46.4%、51.8%和43.6%、61.2%、63.2%,与紫杉醇抑制细胞增殖的活性相近,且在24~72 h范围内具有时间依赖性;化合物2仅在10-5mol/L具有明显抑制细胞增殖的作用,抑制率为44.4%和49.6%。因此,10种不同结构类型的单体化合物中,仅baccatin III类化合物2、4对MCF-7细胞增殖具有较强的抑制作用,其中化合物4作用最强,活性与紫杉醇相近。

Effect of the Compounds from Taxus chinensis var.mairei and T.cuspidata on the Proliferation of Human Breast Cancer Cells

MTT assays were used to study the effects of 10 compounds from Taxus chinensis var.mairei and T.cuspidata on the proliferation of MCF-7 human breast cancer cultured cells in vitro in this study.After treated with compounds(1-10)(10-9-10-5 mol/L) for 48 h and 72 h,compound 4 produced a significant inhibition effect on MCF-7 cells at 10-7-10-5 mol/L(P<0.01),and the inhibitory rate was 29.8%,46.4%,51.8% or 43.6%,61.2%,63.2%,respectively,nearly similar to taxol at the same concentration.During the period of 24-72 h,the inhibition effect was time-dependent.Compound 2 showed a significant inhibition on MCF-7 cells only at 10-5 mol/L (P<0.01),and the inhibitory rate was 44.4% and 49.6%.Consequently,compounds 2 and 4,the analogues of bacctin III,inhibited the proliferation of MCF-7 breast cancer cells significantly,and compound 4 had the higher activity among them,nearly similar to taxol.