血清胸腺因子的固相合成及抗血清的制备

通过Bpoc·Ser(tBu)·Asp·OtBu的β-羧基上树脂和依次用Fmoc·Gly·Gly·OHFmoc·Gln·ONP,Fmoc.Ser(tBu).OH,Fmoc.Lys(Boc)·OH,Fmos·Ala·OH及pGlu·OH伸长,接着氨解和酸解的策略,我们用固相方法合成了血清胸腺因子(FTS,Thymulin),pGlu·Ala·Lys·Ser·Gln·Gly·Gly·Ser·Asn;制备了具有最大生物活性的该因子和Zn~(2+)的复合物,FTS-Zn;用该复合物与牛血清白蛋白(BSA)结合所形成的免疫原免疫家兔,获得了抗FTS-Zn~(++)的抗血清,通过酶联免疫吸附分析法(ELISA)测得其滴度高达1:12800。

Solid Phase Synthesis of Serum Thymic Factor and Preparation of its Anti-serum

By a strategy of attachment of p-carboxyl of Bpoc-Ser(tBu)-Asp-OtBu to the resin support and elongation consecutively wtih Fmoc-Gly-GIy-OH, Fmoc-Gln-ONP, Fmoc-Ser (tBu)-OH, Fmoc-Lys (Boe)-OH, Fmoc-Ala-OH and pGlu-OH, followed by ammonolysis and acidolysis, Serum Thymic Factor (FTS, Thymulin), pGlu-Ala-Lys-Ser-Gln-Gly-Gly-Ser-Asn, was prepared by the solid phase method. The complex of this factor with Zn++, FTS-Zn, which showed maximum biological activity was prepared, conjugated with BSA and immunized the rabbit. The antiserum to FTS-Zn was obtained with a liter of up to 1:12800 by the ELISA method.