苎麻种质资源分子身份证构建的初步研究

以国内外42份苎麻种质资源为材料,采用ISSR引物对供试材料的DNA进行扩增,将琼脂糖凝胶电泳得到的谱带统计结果进行数字化赋值,用自行编制的DNA指纹数据分析器进行数据分析。结果表明,仅需7条引物就可将42份供试材料完全区别开。相对其他DNA分子标记技术而言,ISSR操作简单、检测方便、稳定性好、多态性高,是一种较理想的苎麻分子指纹方法。初步构建了一套包含42份苎麻种质资源的分子身份证。

Establishment of Molecular Identification in Ramie Germplasms

ISSR,an effective molecular marker based on microsatellites sequences,is widely used to genetic diversity and cultivars identification research.In this paper,42 Ramie germplasms collected from 5 countries were identified using ISSR molecular marker.According to fragment size of variation,the data numeralized from the amplified bands were analyzed by the software identification analysis.Seven primers could be used to identify all the 42 Ramie germplasms.The electrophoresis results of each germplasm amplified...