Purification and nucleoside composition of a Q* - containing aspartic acid tRNA from Drosophila. |
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Authors: | M A Wosnick B N White |
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Institution: | The Group in Eukaryotic Molecular Biology and Evolution, Department of Biology, Queen''s University, Kingston, Ontario, Canada |
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Abstract: | One form of aspartic acid tRNA from , (tRNA2δAsp) is selectively bound to columns of Con A-Sepharose. Unlike the other Q-containing tRNAs of , it therefore appears that tRNAAsp contains the more highly modified nucleoside, Q1 (mannose form) in its anticodon. This is further supported by the chromatographic insensitivity of tRNA2δAsp to NaIO4 treatment. Utilizing Con A-Sepharose chromatography, tRNA2δAsp from was purified and its nucleoside composition determined by chemical tritium labelling. In addition to the major nucleosides, this tRNA contains rT, hU, m5C, ψ, and Q1, but no other modified nucleosides. Its nucleoside composition is very similar to yeast tRNAAsp. |
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