Analysis of penicillin-binding sites with a micro method — The binding site of PBP 5 from Escherichia coli |
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| Authors: | Bernd Glauner Wolfgang Keck Uli Schwarz |
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| Institution: | Max-Planck-Institut für Virusforschung, Biochemie II, D-7400 Tübingen, F.R.G. |
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| Abstract: | Abstract A micro method for the isolation and characterization of the penicillin-binding sites in penicillin-binding proteins (PBPs) was developed. Only 10 nmol of a pure PBP are required for the whole procedure which is based on high-pressure liquid chromatography (HPLC). We showed that serine 44 in PBP 5 from Escherichia coli binds penicillin covalently. |
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| Keywords: | PBP-binding sites micro method: PBP 5 E coli |
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