Antibacterial and biofilm removal activity of a podoviridae Staphylococcus aureus bacteriophage SAP-2 and a derived recombinant cell-wall-degrading enzyme |
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Authors: | Jee-Soo Son Se-Jung Lee Soo Youn Jun Seong Jun Yoon Sang Hyeon Kang Hyoung Rok Paik Jung Ok Kang Yun-Jaie Choi |
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Institution: | (1) Laboratory of Animal Cell Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Building 200-#4204, Sillim-9 dong, Seoul, 151-742, South Korea;(2) iNtRON Biotechnology, Inc, Room 703, JungAng Induspia V, 138-6, Sangdaewon-dong, Jungwon-gu, Seongnam-si, Gyeonggi-do, 462-120, South Korea; |
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Abstract: | Antibacterial and biofilm removal activity of a new podoviridae Staphylococcus aureus bacteriophage (SAP-2), which belongs to the φ29-like phage genus of the Podoviridae family, and a cell-wall-degrading enzyme
(SAL-2), which is derived from bacteriophage SAP-2, have been characterized. The cell-wall-degrading enzyme SAL-2 was expressed
in Escherichia coli in a soluble form using a low-temperature culture. The cell-wall-degrading enzyme SAL-2 had specific lytic activity against
S. aureus, including methicillin-resistant strains, and showed a minimum inhibitory concentration of about 1 μg/ml. In addition, this
enzyme showed a broader spectrum of activity within the Staphylococcus genus compared with bacteriophage SAP-2 in its ability to remove the S. aureus biofilms. Thus, the cell-wall-degrading enzyme SAL-2 can be used to prevent and treat biofilm-associated S. aureus infections either on its own or in combination with other cell-wall-degrading enzymes with anti-S. aureus activity. |
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