Protein expression of a self-compatible allele from Lycopersicon peruvianum: introgression and behavior in a self-incompatible background |
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Authors: | Bruce A Rivers Robert Bernatzky |
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Institution: | (1) Department of Plant and Soil Sciences, French Hall, University of Massachusetts, 01003 Amherst, MA, USA |
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Abstract: | Lycopersicon peruvianum (wild tomato) is a gametophytic self-incompatible (SI) species. One natural population has been shown to harbor a self-compatible (SC) allele. A stylar protein associated with the self-compatibility allele has been elucidated using SDS-PAGE. The temporal and spatial expression of this protein is presented and compared with protein expression of two SI alleles. Hybrids containing the SC and SI alleles were used in a backcrossing program to introgress the SC allele into SI backgrounds in six independent lines. Controlled pollinations and SDS-PAGE were used to identify and select classes of progeny. After four backcross generations (approximately 97% recovery of the SI backgrounds) the SC allele still confers self-fertility in lines that contain this allele, providing evidence that the mutation to SC occurred at the S-locus and that the associated protein is likely responsible. |
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Keywords: | Self incompatibility Self compatibility Lycopersicon peruvianum S-related proteins |
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