Determination of cytokinins by mass spectrometry based on stable isotope dilution. |
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| Authors: | T Hashizume T Sugiyama M Imura H T Cory M F Scott J A McCloskey |
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| Affiliation: | 1. Laboratory of Bioorganic Chemistry, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183 Japan;2. Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah 84112 USA |
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| Abstract: | A method for quantitative determination of individual cytokinin species has been developed, based on gas chromatography-mass spectrometry and selected ion monitoring. Deuterated internal standards were prepared for analysis of N6-isopentenyladenosine, N6-isopentenyl-2-methylthioadenosine, zeatin riboside, and 2-methylthiozeatin riboside and were tested over the range of 1 to 20 ng of endogenous cytokinin per injection, relative to 100 ng of labeled standard. An isolation procedure for extracts of cabbage hearts as a model plant source has been developed that gives maximum recovery and minimum interference for gas chromatographic-mass spectrometric measurements. The present method differs from the commonly used bioassay by its selectivity for individual cytokinin components and shortened analysis time, including extractions, of 3 days vs several weeks. |
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| Keywords: | To whom correspondence should be sent. |
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