Modulation of T-cell functions: I. Effect of 2-mercaptoethanol and macrophages on T-cell proliferation |
| |
Authors: | Randolph J Noelle David A Lawrence |
| |
Institution: | Department of Microbiology and Immunology, Albany Medical College of Union University, Albany, New York 12208 USA |
| |
Abstract: | The in vitro effects of 2-mercaptoethanol (2-ME), macrophages (MØ), and concanavalin A (Con A) on the proliferation of normal spleen cells (NSC), MØ-depleted spleen cells (DSC), T cells, T-cell subpopulations, and B cells were assessed by 3H]thymidine incorporation. 2-ME alone was consistently shown not to be mitogenic for purified T cells; however, 2-ME enhanced the early (Days 1 and 2) Con A (2 μg/ml)-induced response of NSC, DSC, and T-cell preparations, but depressed the late response (Days 4 and 5). 2-ME alone was mitogenic for purified B-cells, as reported previously; and the 2-ME-induced B-cell response was inhibited by Con A. Preincubation of T cells with 2-ME was sufficient for enhanced Con A responsiveness; however, if 2-ME was added 24 hr after the initiation of culture, no alteration of the Con A-induced response was observed. Ly-2,3+ T cells were unresponsive to Con A (0.3–20 μg/ml), but the addition of 2-ME or peritoneal cells enhanced the Con A responsiveness of Ly-2,3+ T cells over 200-fold. Ly-1+ T cells responded with a similar doseresponse and kinetic profile as unselected T cells. Although Ly-1+ T cells responded to Con A, unlike Ly-2, 3+ T cells, extensive removal of MØ significantly reduced the Con A-induced responsiveness of the Ly-1+ T cells. The reactivities of Ly-1+ and Ly-2,3+ DSC could be reconstituted by the addition of MØ or 2-ME; however, the kinetic response of Ly-1+ T cells peaked on Day 2–3, and Ly-2,3+ T cells had a delayed response which peaked on Day 4–5. The results indicated that (i) 2-ME and/or MØ accelerate the response kinetics of T-cells to Con A; (ii) T-cell subpopulations have differential requirements for MØ and/or 2-ME in the response to Con A; (iii) T-cell subpopulations exhibit differential dose responsiveness to Con A; and (iiii) 2-ME alters Con A responsiveness by a direct effect on T cells. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|