丙型肝炎病毒核心区优势抗原表位基因的化学合成及其表达产物的抗原性分析

依据丙型肝炎病毒（ＨＣＶ）多蛋白核心区Ｎ端氨基酸序列和密码子简并性，人为设计并采用半化学半酶促的方法合成了一个ＤＮＡ片段。经核酸杂交检测以及ＤＮＡ序列分析证实，该片段的核苷酸序列与设计完全一致。将合成的ＤＮＡ片段插入融合表达载体ｐＧＥＸ－２Ｔ中，表达产物经亲和层析纯化后进行Ｗｅｓｔｅｒｎ免疫印迹实验和间接ＥＬＩＳＡ分析，结果表明，融合蛋白具有ＨＣＶ核心区抗原的免疫反应性，可望用于ＨＣＶ抗体的检测．此外，编码ＨＣＶ优势抗原表位的化学合成基因有可能为ＨＣＶ嵌合抗原的研究提供一条捷径。

Synthesis of DNA Fragment Encoding for Dominant Epitopes of Core Region of Hepatitis C Virus(HCV) and Antigenic Analysis of Recombinant Protein

The synthetic DNA encoding for N-terminal dominant epitopes of core region of HCV polyprotein was synthesized with a semi-chemical and semi-enzymatic method and identified through DNA sequencing. The DNA fragment was inserted into the prokaryotic expression vector pGEX-ZT to express fusion protein GST-C74. Afer purified by affinity purification,the product was analysed with Western blot and indirect ELISA, the results showed that GST-C74 has the similar antigenity as core antigen as expected,thus it could be used in anti-HCV immunoassay. On the other hand,synthetic DNA fragment coding HCV dominant epitopes will be beneficial to the research of chimeric antigens.